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Because GFRα1 is specifically expressed in As, and Apr undifferentiated spermatogonia in vivo and, therefore, the GFRα1+ population did not express Kit [3], we also checked expression patterns of GFRα1 at the protein level by flow cytometry.
We also checked expression of genes that impact on the H2B-Ub-H3K79-H3K4 pathway, Rnf20, Ube2e1, Dot1l and Mll1: none were altered by the YA-DN (Fig. 3).
We next checked expression of the three candidates in preimplantation embryos.
We checked expression of selected haploid-specific genes in all E6 and E15 derived clones.
(22) We checked expression of ROCK1 in MKN-1 and the alteration of its expression in miR-148a-deregulated cells.
We also checked expression of NR4A3 (also called Neuron-derived orphan receptor (Nor) 1) by RT-PCR in our models.
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When checked expressions of the NSCs markers (Nestin; Sox2), differentiated neuronal markers (Neurod1; ßIII-tubulin) and glial marker (Gfap), the expression of both Nestin (B) and Neurod1 (C) were significantly down-regulated following exposure to the HMF.
Anatomical meta-expression data from ROAD were used to check expression patterns in other tissues.
Encouraged by the study results, the team suggests that it may now be technologically possible to screen for cancer by checking expression levels of bronchial airway cell genes.
As a control, all of the Affymetrix anatomical meta-expression profiles in the Rice Oligonucleotide Array Database (ROAD;), except for two anther samples, were used to check expression patterns in other tissues/organs (Cao et al. 2012).
To check expression of receptors and TPR-proteins replicate lysates were pooled, briefly sonicated and cleared by centrifugation.
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