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Further, normal keratinocyte (HaCaT) and osteosarcoma (Saos-2) cells seeded on PVA-Car scaffolds were cryopreserved for 15 days and their viability was checked at regular interval of 3 days (0, 3, 6, 9, 12, 15 days) through MTT assay and fluorescence microscopy.
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After counseling, women signing Institutional Review Board–approved informed consent had their methadone serum trough 20-244 hours after last dose) level checked at regular intervals throughout pregnancy.
She was checked at regular intervals after returning to the ward, but at about 07 30 BST she was unresponsive and her condition deteriorated.
All hammer and tool heads must be securely fastened and checked at regular intervals.
Aliquots of master mix were checked at regular intervals till seven months for the PCR assay.
To assess the reliability of data collection, interobserver variability was checked at regular intervals.
The BHI was refreshed daily to ensure the growth of SM on the dentin blocks, and the purity of the cultures was checked at regular intervals.
Hepatotoxicity should be suspected in any patient with gastrointestinal disturbance and liver enzymes must be checked at regular intervals [ 185] (table 6).
The reducing sugar level in treated sample was checked at regular intervals to study the effect of enzyme on the pectin degradation from banana fibers.
Ideally, seroconversion status should be ascertained prior to pregnancy, and if seronegative, clients need to be checked at regular intervals during pregnancy with the obvious disadvantages of generating anxiety and misinterpretation of test results.
All trees were checked at regular intervals to ensure all stem growth was maintained in the correct growth orientation i.e. 45° or vertical, and to minimise the impact of the gravitropic response (in the tipped trees) of the apical meristem returning to vertical growth.
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CEO of Professional Science Editing for Scientists @ prosciediting.com