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After PCR products cleanup using 0.8x volumes AMPure XP beads, amplicons length and amount were checked as described above.
Survival was checked as described above.
Activity of the mutated protein was checked as described in section 2.5.
Mice were monitored for pregnancy outcome and cytokines profiles were checked as described in material and methods section.
Genotypes were automatically called with the SpectroCALLER software (Sequenom), and manually checked as described in Silander et al [32].
The purity and integrity of the RNA were checked as described previously [ 16].
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Posterior predictive simulation was used for model checking as described by Gilks et al. [ 28].
We analysed knowledge of symptoms and the increasing risk of breast cancer with age, and reported breast checking as described in our previous publication (Linsell et al, 2010).
All sequences were subject to sequencing, assembly, finishing and checking as described [ 39], with annotation facilitated by the programs Artemis [ 40] and ACT [ 41].
The survey featured the Moral Foundations Questionnaire, Liberty Foundation Questionnaire and two attention checks as described in study 1.
Students with Teaching Appointments: Students who are appointed Teaching Fellow or Research Fellow also receive 4 stipend checks as described above which equals 2/3 of their total stipend.
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