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"That check was stained with my tears".
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"Cleanliness, not having menstruation every month, when I go out I don't have to check out if my clothes are stained or not".
Cells were digested and seeded in 6-well plates and 1000 cells were stained and checked for cell viability rate.
For checking apoptotic cell death, cells were stained with Annexin-V FITC and propidium iodide (PI) after treatment with C2 and photoirradiation.
In all, 12 mattresses were checked by trust staff and 11 were stained through to the foam.
Their viability was then checked with trypan blue and they were stained with 2 µl mAbs within 20 minutes.
Keep checking on the shorts until they are stained enough.
The enrichment in EBV- or HCMV-specific T cells was checked by staining by ad hoc pMHC tetramers, and two successive sortings were made, when necessary, to achieve a purity between 89 to 100% (Table 1, Fig. 1.A, and data not shown).
Slides were washed in buffers 1 and 3 (100 mM NaCl + 100 mM Tris+ 50 mM MgCl2, pH 9.5) for 5 min. Substrate (NBT + BCIP) was added and slides were checked for staining.
Zone of clearance within the gel was checked after staining with Coomassie brilliant blue R-250.
Purity of the myoblast preparation was checked by staining with an anti-desmin antibody revealing more than 95% desmin-positive cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com