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Simultaneously, sample weight loss was measured by TGA to check the preparation procedure and determine the effective water content (error ≤ 1 wt%).
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The amount of total RNA obtained was high enough to directly check the quality of the preparation by spectrophotometry, followed by conventional electrophoresis.
Algorithm 1 incorporates items to check during the preparation, planning and delivering of anaesthesia, to supplement the World Health Organization checklists.
We also checked the CRP preparation by polymerase chain reaction (PCR) technique for potential DNA contamination [ 30].
In order to examine the transcripts levels between olfactory and non-factory tissues, amplification of a "house-keeping" gene encoding actin was used as control to check the integrity of each cDNA preparation.
In order to examine the transcripts levels between olfactory and non-olfactory tissues, specific primers of a "housekeeping" gene encoding ribosomal protein L8 (CquiRpL8) were used as control to check the integrity of each cDNA preparation.
In order to be transposed to C-S-H, this would first require acquiring XRD patterns from an oriented preparation to check the rationality of 00 l reflections.
In order to check the homogeneity of clusters in preparation for a planned cluster randomized trial we conducted a baseline survey.
It is of note that all these reagents were used as positive control to check the phenotype of the cell preparations.
We also aimed to amplify the full open-reading frame of the chitin synthase gene mcs1, previously used to check the quality of our RNA preparations (see above).
To check the purity of the tPA preparations, SDS-PAGE was performed using a 5% stacking gel and a 10% separating gel in Tris-glycine running buffer.
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