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First, it provides an anchor for primers when these are used to check the orientation of the inserted transgenes.
Check the orientation of the assembly and its contacts.
Check the orientation of the fuse holder by looking for the ON and / or OFF indicators in the middle of the short sides of the fuse holder.
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In addition, we also checked the orientation angles of the subjects' phones that were recorded when they were walking down these same paths and same directions.
We then PCR amplified the cho-1 promoter with the primers 5'-TCCCCGCGGATACTTCAATTATTCCCGTCTTACCAGG-3' and 5'-TCCCCGCGGTAAGATAGAGGAACTCGCAAGATGTCG-3-3', digested with SacII, and cloned into the SacII site carried over to pBR322 and checked the orientation.
Graphs were generated for a preliminary view of the assembly (Fig. 1) in an effort to check the order and orientation of mitochondrial scaffolds in the genome.
Check the map's orientation.
To further check the location and the orientation of the LDM fibers ultrasonography was performed by ultrasound probe (Carsi Plus ESAOTE, 7.5 10 MHz probe) first with the muscle relaxed (muscle thickness 0.5 0.8 cm) and then during a maximum voluntary isometric contraction (muscle thickness 0.7 1.8 cm).
After checking the correct orientation, the IRES-BSD transgene transcription unit could be isolated with or without MARS element from the respective vectors as BglII and MluI fragments.
Hence, in 2013, the second edition was updated and radically refined: we checked the theoretical orientation of each trial and all included trials were recategorised, and data completely re-extracted and reanalysed based on baseline never-smoking cohorts.
But tc is rather sensitive to the substrate orientation, as shown by many studies that have been conducted to check the effect of substrate orientation on tc [18, 19].
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com