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We derived a Perl program to check the occurrence of peptides upstream of the PTC.
Primers P1 (5'-ATCTCCACTGACGTAAGGGATGAC-3') and P2 (5'-GTTTAAGATCTACTTACGTAATCAAGC-3') were used to check the occurrence of marker excision events.
Thus, we decided to check the occurrence of this event by the presence of LC3, a well-known marker of autophagosome formation.
This observation offers the possibility to check the occurrence of these correlations also in proteins whose structures were generally determined at moderate resolution such as membrane proteins.
Given our initial results regarding the importance of AMBRA1 LIR domain in mitophagy (see Figures 1c and d), we decided to check the occurrence of AMBRA1 ActA/LC3 interaction through this domain.
All patients were followed until March 2008 to verify the maintenance of the aesthetic result, but only 64 were further monitored over time until March 2009 (2 years follow-up) so as to check the occurrence of some unexpected event after the total absorption of the CMC.
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Moreover, we also designed a simple protocol to identify scaffolds by checking the occurrence of cyclic substructures, including simple rings, ring assemblies, and bridge assemblies.
Each of these indictor function checks the occurrence of a feature.
We checked the occurrence of each of these references in the search results of the corresponding Google Scholar search.
In addition specific rules for checking the occurrence of these delays were developed and systematically used for all cases.
This review was performed by two nephrologists (HJ and HJGB) who independently checked the occurrence of ADEs.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com