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To check the localisation of the mutant protein, a GFP fused version was expressed in zrc1Δ.
To check the localisation of the tagged SOD A, epimastigotes were fixed in 4% paraformaldehyde and dried onto slides.
To check the localisation of the MR-sensitive volume, we used the same experimental set-up to obtain H images and estimated that nearby liver tissue represented less than 5% of the detected volume.
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The antibodies were used to check the subcellular localisation of the TTC4 protein using indirect immunofluoresence in the melanocyte lines Nohm 1 and Hermes 3a (Fig 6a/b).
To check that the localisation was not an artifact of tagging or over-expression, we examined V5-PUF7 in the in situ tagged procyclic forms.
We then checked the co-localisation of p53 isoforms with polg.
Fifteen minutes after injection, the embryos were fixed, and the localisation of the bacteria was checked by using transmission electron microscopy (TEM).
If such factors influence the precision of the localisation responses, we should see localisation errors that nonetheless cluster around the correct region.
The localisation movement never tends to do that, they just say localisation is a great idea, it's sustainable, it's good for the community.
The localisation movement has not always been good at talking about economics, Hopkins says.
This week, the Guardian exposed a worrying development in the localisation of the discretionary payments of the social fund.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com