To further check the expression of pGPR120 in the corresponding established cell lines related to the highly expressed tissues, we subsequently adopted a qPCR strategy to examine the expression of total pGPR120 in various cell lines and primary cultured cells.
"I think that we should be eternally vigilant," he added, "against attempts to check the expression of opinions that we loathe and believe to be fraught with death".
As Justice Holmes once said, "we should be eternally vigilant against attempts to check the expression of opinions that we loathe".
I'm with Justice Oliver Wendell Holmes Jr., who wrote in 1919 that: "I think we should be eternally vigilant against attempts to check the expression of opinions that we loathe and believe to be fraught with death".
We want to check the expression derived by Van Nee in [8] for the conventional OFDM for the discrete case.
We consider the OFDM/OQAM as being an example of the FBMC system, and we check the expression derived by Skrzypczak [22].
1 μL of cDNAs were used as template to run 20 μL real-time PCR reactions to check the expression of house-keeping gene GAPDH and other genes to be detected, including CD133, GFAP, Dlx2, Dcx, Ncam1, Mapk1, and Mapk3.
The primers qRT-1 F and qRT-1R were used to check the expression levels of OsHsp18.0-CI in the OsHsp18.0-CI-OE lines, and for the OsHsp18.0-CI -RNAi plants, the primers qRT-2 F and qRT-2R were used.
should be eternally vigilant against attempts to check the expression of opinions that we loathe and believe to be fraught with death, unless they so imminently threaten immediate interference with the lawful and pressing purposes of the law that an immediate check is required to save the country.
While that experiment is part of our system, I think that we should be eternally vigilant against attempts to check the expression of opinions that we loathe and believe to be fraught with death, unless they so imminently threaten immediate interference with the lawful and pressing purposes of the law that an immediate check is required to save the country.
RNA was extracted from an overnight bacterial culture to check the expression of lacI and from intracellular bacteria (isolated from RAW 264.7 cells) to check the expression of SPI-2 genes.
