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To check the enzyme stability, enzyme was pre-incubated for 1 h at room temperature in the presence of 5% (v/v) of the selected solvents, and remaining activity was measured spectrophotometrically.
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It is very convenient for a user to check the available restriction enzyme for each gene of interest, both online and per email.
7) SDS-PAGE analysis SDS-PAGE analysis [ 28] was performed following the method of Laemmli with a 10.0% separating gel to check the purity of the enzyme sample obtained by affinity precipitation.
In order to check the efficiency of the enzyme-labeled nanobody, we coated the H3N2 antigen (5 μg/mL, 100 μL) and used the nanobodies coupling with HRP (10 μg/mL, 100 mL) as detector in our ELISA experiment.
To check the structural flexibility of the enzyme in both aqueous and nonaqueous environments, further simulation is needed to compare the structural basis of the enzyme.
To check the thermostability, the crude enzyme was incubated at temperatures ranging from 70 °C to 90 °C, and after incubation for different time periods viz.
It would be interesting to check the activity of truncated enzyme containing only the CHAPk domain with modified amino acid residue at position 114.
This procedure could also be employed to check the effect of variation in enzyme kinetic parameters in the presence of external inhibitors.
To check the relationship between acid production and enzymes activities several tests were done.
The libraries were validated by enzyme digestion in order to check the diversity of clones obtained.
To verify the requirements of LTA4H for substrates with unblocked α-amino group (a vital feature for aminopeptidases) we checked the ability of this enzyme to recognize substrates with secondary amine derivatives (Pro, Tic, Oic) and with modified main chain (Apns (2S,3S), β-Ala, 6-Ahx).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com