We further checked the differentiation states of parental cells and sublines because drug sensitivity can reportedly be affected by differentiation (Costello et al, 2000; Ohashi et al, 2000).
In the following experiments, we used 2BP at the concentration of 10 to100 µM in osteoblasts to inhibit PAT activities and check the consequences in two aspects: differentiation and mineralization.
Different gene markers that are important for brain and retina formation and neuronal differentiation were used to check the phenotypic changes after knockdown of zfPRL2 by morpholino (MO) in zebrafish embryos (Table S2).
Check the spark, Check the carburetor, and check the compression.
To examine whether CDCA mediates differentiation through FXR signaling,we checked the mRNA expression of FXR in CDCA-induced and differentiated mES cells.
In parallel with the analysis of the energetic metabolism, we also checked the change in protein expression taking place during the differentiation process.
Toluidine blue, safranin O staining and real-time PCR were performed to check chondrogenic differentiation.
To check for primary differentiation to the TE, 45 genes which have been previously reported to be involved in its differentiation were investigated in all the single blastomeres from the 5- to 8-cell stage embryos [1], [2], [18], [25], [33], [36], [40], [45], [49] [51] (Table S2).
To determine alterations in a differentiation and proliferation-related transcription factor in the brain, we checked the expression of tailless (TLX) in mice fed a HFD.
We have performed additional experiments to check whether AA induces the differentiation of hUCB-MSCs in vitro.
