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A P-value is calculated using the one-tailed Fisher's exact test to assess whether N AC /N C > N AI /N I and annotation term A is enriched in cluster C. Such functionality is very useful to check the cluster quality at different identity levels and also for function assignment of proteins with unknown function.
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HD and 50k genotypes were used for parentage testing and to check the clustering quality of the HD genotypes by concordance analysis of the genotypes of the 1838 individuals genotyped on both chips.
We further checked the cluster density, i.e. the average number of ones in all binary vectors in each cluster, and selected the eight largest clusters (R1 to R8) in terms of the cluster density.
This module includes functions responsible for checking the cluster status and terminating the cluster.
In order to verify our subdivision of the GH97 family into subfamilies we checked the clustering of the family members in the phylogenetic tree.
We then expanded the clusters by 500 bp windows if they contained 1 piRNA and checked the clusters that had expanded by more than 20%% of their length.
Preliminary identification of clusters of orthologs of RR and HK sequences was performed by creating an orthology table of the 19 genomes used in this study using the clustering algorithm implemented in MBGD [ 43] and manually checking the clusters of orthologs thus obtained for each previously identified TCS gene.
When clustering analysis is performed, we need a way to represent the resulting clusters in the tree, and to be able to interact with these clusters to check the accuracy of the clusters.
To check the robustness of the clusters, clustering of samples was repeated with the Covariance and Cosine correlation distance metrics.
The routing from S to G1 is performed within the cluster by checking the intra-cluster routing tables.
The cluster heads then check the status of their cluster members.
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