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The field hand-over test of equipment is required to check the assembly quality of equipment following field installation and before being put into operation to evaluate the equipment performance.
To check the assembly result, nine partially overlapped fragments covering the entire C. oliveri cp genome were amplified and sequenced.
The PCR to check the assembly was conducted using the TaKaRa Ex Taq system and the primer pairs used are listed in Table 1.
Check the assembly at this point to make sure it is square.
If only some of the keys do not perform correctly, check the assembly near them in particular.
SL, MS and MPK contributed mapping information and examined linkage data for all autosomes to quality check the assembly.
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PacBio reads, used to check the assemblies, were downloaded from http://gembox.cbcb.umd.edu/mhap/raw/dmel_filtered.fastq.gz.fastq.gz
He checked the assembly line, met with his board and made certain all the American flags were in place and the ribs and catfish ready to barbecue.
Checking the assembly ACE file, we found that no read can bridge the two neighboring contigs long enough for them to be connected by assembler.
Finally, the panel proved useful for checking the assembly of sequence scaffolds and for mapping EST located on one of the smallest microchromosomes.
In addition to the expected advances in fine-mapping of Quantitative Trait Loci (QTL) [ 1, 2], high-density linkage maps provide a framework for checking the assembly of genome sequences and for studies of the evolution of these genomes through the analysis of recombination.
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