Protein structural analysis with these amino acid variants was performed by using I-Mutant, FOLD-X, HOPE, NetSurfP, Swiss PDB viewer, Chimera and NOMAD-Ref servers to check their solvent accessibility, molecular dynamics and energy minimization calculations.
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The blend co-continuity was checked by solvent extraction technique.
The sensing mechanism was systematically investigated by employing high resolution mass spectrometry, 1H NMR and density functional theory calculations as well as checking the solvent viscosity dependent fluorescence, and thus the nucleophilic addition products, the intramolecular charge transfer character, and the aggregation-induced emission behaviour were clearly elucidated.
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The trick is keeping your vices in check, your checkbook solvent and ignoring the insiders' tips that waft their way from comfortable taverns downtown and on through the paddock and grandstands.
Blank matrices along with reagent blank were run during validation to ensure minimal risk of interferences, guarantee specificity of the method and to check for potential solvent contamination.
For this task, methanol, tetrahydrofuran, acetonitrile, dichoromethane and chloroform were checked as washing solvent, in which chloroform was able to establish the specific interaction between catechol and those sites of molecularly-imprinted polymers (MIP).
Identical instrument settings for the sample and standard solutions were carefully checked, and the solvent adsorption and emission spectra were subtracted from the absorption and emission spectra of the sample and standard solutions.
In order to check the absence of solvent's toxicity, the comparison of Fas protein levels in group C (sham operated rats) and group DMSO (sham operated rats with use of solvent DMSO intraperitoneally) was performed first.
