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Spot check sampling and analyses of sterile swab tests from 18 animals at different time points after open osteotomy (i.e. at 29, 42 and 84 d) revealed positive results for S. aureus in 4 animals.
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In vitro tests were performed to check samples cytotoxicity and the behavior of osteoblast-like SaOS-2 cells.
(g) Check sample: Check sample by re-runing mid point calibration curve in middle and at end of sample sequence.
Fish Canyon Tuff zircons (Gleadow et al. 2015) were also run as "unknowns" with each batch of samples to check sample accuracy.
Periodically, check samples were analysed to detect potential problems with the reagents or the spectrophotometer.
As such it is important to check sample veracity prior to analysis.
Calibration check sample results were used to assess stability of the instrument calibration.
No check samples should deviate more than 10% from the amount of nitrite of the matching calibration standards.
In the meantime, for banked samples previously collected for the measurement of global histone marks, Western blot can be used to check samples for enzymatic cleavage of histones.
For 94 subjects, there was adequate sample to prepare and analyse at least two replicate subsamples to check sample homogeneity and analytical reproducibility.
Corresponding FMeHg QAQC samples included field/method blanks (less than detection in all cases), duplicate analyses (%RSD) = 4.6%), matrix spikes (recovery = 97.1% ± 21.6%), and check samples.
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