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In vitro tests were performed to check samples cytotoxicity and the behavior of osteoblast-like SaOS-2 cells.
Periodically, check samples were analysed to detect potential problems with the reagents or the spectrophotometer.
No check samples should deviate more than 10% from the amount of nitrite of the matching calibration standards.
Corresponding FMeHg QAQC samples included field/method blanks (less than detection in all cases), duplicate analyses (%RSD) = 4.6%), matrix spikes (recovery = 97.1% ± 21.6%), and check samples.
In the meantime, for banked samples previously collected for the measurement of global histone marks, Western blot can be used to check samples for enzymatic cleavage of histones.
The system suitability check samples or quality control samples prepared in vehicle should be injected periodically throughout every chromatographic run to assess consistent analytical performance and serve as a performance check.
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(g) Check sample: Check sample by re-runing mid point calibration curve in middle and at end of sample sequence.
Fish Canyon Tuff zircons (Gleadow et al. 2015) were also run as "unknowns" with each batch of samples to check sample accuracy.
As such it is important to check sample veracity prior to analysis.
Calibration check sample results were used to assess stability of the instrument calibration.
For 94 subjects, there was adequate sample to prepare and analyse at least two replicate subsamples to check sample homogeneity and analytical reproducibility.
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