Two pairs of primers (Gox Fw1-Rv1 and Fw2-Rv2) amplified the internal sequence of the putative gox gene, and the third pair of primers (Gox Fw3-Rv3) was designed to amplify the putative gox gene together with the flanking regions in order to check the replacement of the gox gene (Figure 1a).
Check the replacement neck's fit within the pocket.
In situations where the cells are checked for replacement at discrete intervals, referred to as maintenance event intervals, it is found that the length of the interval is dependent on the mean time to failure of the individual cells.
The tetracycline-resistant transformants were screened by PCR using appropriate gene specific primers, for checking the replacement of wild-type genes on chromosome.
Strategies developed to complement the nurse care manager role focused on asking patients to directly contact nurse care managers (such as for referrals for blood pressure checks), nicotine replacement therapy review, consults to resources, and any other medical concerns.
However, those traps placed 20 meters high would have had to be lowered to the ground daily for checking and replacement of the dry ice, and that would give ticks a chance to crawl onto the traps out of their own volition.
This proved to be a faster and more accurate way of making the changes, while still manually checking each replacement, than to load this set of Jigsaw predictions into the curation tool where the required changes to the gene structure would then have needed to be constructed manually.
Worley sent frantic e-mails and made repeated calls to Nigeria, begging for a replacement check.
She got a check to cover replacement costs and thought that was the end of it.
Do we bring this up and ask for a replacement check, or let it go?
