Sentence examples for check primer from inspiring English sources

To check primer specificity real-time PCR was performed on cDNA and products were analyzed on 3% SYBR Green staining agarose gel and by melting curves.

Suggestion: Check primer dilutions, you may need to set up fresh working dilutions of primers from oligomer stock.

Oligocalc was used to check primer candidates for self-complementarity and to ensure that pairs of primers had similar melting temperatures (Oligo Calculator version 3.23).

The thermal cycling conditions were as follows: 1 cycle (10 min at 95 °C) followed by 45 cycles (10 s at 95 °C, 10 s at 59 °C and 15 s at 72 °C) followed by a melting curve analysis to check primer specificity.

Primer sequences were used as queries in a BLAST search to check primer specificity against the common bean genome sequence database.> For CAPS marker (Table  5), the SNP was analyzed for potential restriction enzyme recognition sites using the SGN CAPS designer (http://sgn.cornell.edu/tools/caps_designer/caps_input.pl).edu/tools/caps_designer/caps_input.pl

PCR amplification using genomic DNA of nulli-tetrasomic lines (NA-TD, NB-TD, and ND-TB) in Chinese Spring wheat was used to check primer specificity and to determine which EPSPS gene was located on which wheat chromosome (Table  1; Additional file 1).

Successfully recombination was determined by antibiotic selection and loci screening using check primers (Table 2).

Genomic DNA shows the results of PCR with genomic DNA as a positive control to check primers.

Antibiotic resistance was removed by the expression of FLP recombinase from the pCP20 plasmid, and successful deletions were confirmed by loss of antibiotic resistance and by PCR using loci check primers (Table 2).

A non-template control sample was used for each primer to check primer-dimer formation.

However, the check for primer similarity (using AUTODIMER software) is of limited utility when checking primers containing degenerate bases because the degenerate bases are regarded as unknown bases and some unidentified primer pairs may turn out to be incompatible.