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This is why you should make it a habit to check orientation of coins every time you hold one with the simple over-the-top-turn technique.
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Moreover, the alternative checked orientations did not satisfy the spatial restrains on the distance between neighbouring subunits.
If this sounds familiar, you may need to check your orientation.
First, it provides an anchor for primers when these are used to check the orientation of the inserted transgenes.
Check the orientation of the assembly and its contacts.
Check the orientation of the fuse holder by looking for the ON and / or OFF indicators in the middle of the short sides of the fuse holder.
Weissenberg multiple-film techniques were used in visual evaluation of the reflexion intensities, and the precession camera was used in determining the cell dimensions and in checking the orientation adopted.
In addition, we also checked the orientation angles of the subjects' phones that were recorded when they were walking down these same paths and same directions.
Each fragment was checked for orientation by using KS2808 and the fragment-specific reverse primer.
Samples were checked for orientation and inclusion of AF and NP, and scored for degree of degeneration using a published histological 12 point scale [ 24].
We then PCR amplified the cho-1 promoter with the primers 5'-TCCCCGCGGATACTTCAATTATTCCCGTCTTACCAGG-3' and 5'-TCCCCGCGGTAAGATAGAGGAACTCGCAAGATGTCG-3-3', digested with SacII, and cloned into the SacII site carried over to pBR322 and checked the orientation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com