The authors would like to thank D. Benson, D. Burge and P. Rymer for useful discussions; and R Milner and J Baum for collection support and N. Weston and J. Siow for checking occurrence records.
Primers P1 (5'-ATCTCCACTGACGTAAGGGATGAC-3') and P2 (5'-GTTTAAGATCTACTTACGTAATCAAGC-3') were used to check the occurrence of marker excision events.
Thus, we decided to check the occurrence of this event by the presence of LC3, a well-known marker of autophagosome formation.
This observation offers the possibility to check the occurrence of these correlations also in proteins whose structures were generally determined at moderate resolution such as membrane proteins.
Given our initial results regarding the importance of AMBRA1 LIR domain in mitophagy (see Figures 1c and d), we decided to check the occurrence of AMBRA1 ActA/LC3 interaction through this domain.
All patients were followed until March 2008 to verify the maintenance of the aesthetic result, but only 64 were further monitored over time until March 2009 (2 years follow-up) so as to check the occurrence of some unexpected event after the total absorption of the CMC.
Note that the normalized demand function introduced in Eq. (7) checks the occurrence of failure at two specific times only instead of checking failure at each time t ∈ [0, t F ]. Nonetheless, this strategy is still valid due to the fact that in this contribution, cracks are artificially propagated beyond their critical length and that the crack length is a function increasing with time.
Moreover, we also designed a simple protocol to identify scaffolds by checking the occurrence of cyclic substructures, including simple rings, ring assemblies, and bridge assemblies.
These records where assigned the quality label G. Subsequently, in step 3, the workflow performs an environmental congruence analysis: it checks that occurrence records are not located in the sea or lakes.
