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Transformed bacteria were applied to LB plates containing 50 μg ml-1 ampicillin for selection and 40 μg ml-1 X-gal for detection of α-complementation [ 77]. White and positive colonies were picked for colony PCR screening to check inserts.
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PHP scripts call R functions to check, insert and format data, and to perform online statistical analysis or visualisation.
10 μl PCRs with M13 forward and reverse primers were carried out to check insert size and the presence of a single insert and, from these, 96 colonies were chosen for 100 μl PCRs.
Just check the inserts in your Sunday paper and see which brands AT&T and Verizon are offering up these days.
Users can also display, edit or create charts, insert formulas, change formatting, spell check, or insert comments into any Microsoft Word, Excel, or PowerPoint document.
We need a simple clothes button, and a Rubber band (preferably the same colour) Insert the Rubber band through button holes (check images) Insert one end through the loop of rubber band's other end Tighten the knot The final product is here.
The shoes were metallic wedges and the nearest thing to a Burberry bag was its check pattern inserted into gilded leather.
You can check by inserting a knife or prodding it with the back of a spoon – the fish should have a little bounce, but also flake when you apply more pressure.
Most prominently, they disagree on the appropriateness of Southern Blotting as an exhaustive method to check the insert's stability and targeted comparative analyses as a method to detect potential hazards.
Selected colonies were checked for inserts by colony PCR with T7 and Sp6 primers (Fermentas, Hanover, MD).
R [ 16] functions check and insert them into the database.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com