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After ESI-MS analysis to check for successful oxidation, the peptide was column purified and lyophilized.
Furthermore, we will use the CEQ-data to check for successful blinding of participants.
The PCR products were electrophoretically separated on 2% agarose gels and stained with ethidium bromide to check for successful amplification.
A minus RT control with primers for GAPDH was performed to check for successful removal of all the contaminating DNA.
To check for successful deletion, we performed two different control PCRs, using primers csfDEL5F and csfDEL3R as well as ENVSP1F and ENVNEU2R (for primer sequences see Table 1).
The rats (n=5 in each group) were anesthetized by 3% isoflurane; loss of righting reflex, unresponsiveness and corneal reflexes were used to check for successful anesthesia.
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For frames, for which an acknowledgement is expected, it sets a respective timeout and checks for successful delivery.
PCR reaction mixtures were initially checked for successful amplification on a 1.5 % agarose gel stained with ethidium bromide, and viewed under UV light.
The PCR products were cleaned with the Invisorb Spin PCRapid Kit (Invitek) or Quick-Clean (Bioline) and checked for successful amplification by running an aliquot on a 1.5% agarose gel, stained with 1.3 × 10-4 mg/ml ethidium bromide.
Membranes were checked for successful protein transfer and loading control by incubation of the membranes for 1 min in ponceau S. The MS-MLPA is a semi-quantitative method for methylation profiling using a methylation-sensitive restriction enzyme (Nygren et al, 2005).
Because the whole inserted fragment is too long to amplify from genomic DNA, the over-expressed genes were checked by two reactions for successful insertion and correct orientation of the slr1609 or FAR gene and complete replacement of wild-type alleles.
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CEO of Professional Science Editing for Scientists @ prosciediting.com