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To check for positive selection on the other 18 branches in the tree, a Branch-Sites analysis was carried out.
Some cells were retained to perform immunofluorescence (IFA) directed at RVFV to check for positive cultures.
Check for positive hair follicles, i.e. the presence of blue staining of the base of the hair follicles, using a stereomicroscope (Fig. 3).
Resulting colonies (n = 288) were isolated and analyzed to check for positive clones and to choose clones with different restriction profiles.
Although we were primarily interested in the signs of positive selection in the last 5 6 million years of human evolution, it is interesting to check for positive selection on Cernunnos-XLF in more recent times.
Check for positive feedback.
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Then, validation was carried out by checking for positive correlation between the average simulated interaction rate (left( {I_{i}^{text{s}} } right)) and the expected value of the observed interaction rate (left( {I_{i}^{text{o}} } right)).
Similarly, inoculated MGIT tubes were also checked for positive fluorescence weekly until 8th weeks.
The samples were checked for positive coagulase reaction after 2, 4, 10, and 24 h.
The MGIT 960 was checked daily for positive and negative cultures and LJ cultures were checked at least weekly.
PCR amplification to check for the positive clones, i.e., to confirm the presence of insert, and to generate sequencing templates was performed as previously described [ 31].
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