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For MER and macro-test stimulation, some extra time was needed to attach the microdrive for the microelectrodes, connect the wires to the needles, and check for integrity of the system and stability of the signals.
For ontology curators to maintain accuracy and consistency in a structure this large and complicated, it is critical to incorporate automated methods to check for integrity and to help build the ontology graph [ 17- 19].
RNA quality was assessed by the 260/280 nm ratio as well as gel electrophoresis on a 1% agarose gel stained with 1× SYBR Green I (Invitrogen) to check for integrity of the 18S and 28S rRNA bands.
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The downloaded data were checked for integrity before and after merging.
dsRNA preparations were checked for integrity on an agarose gels stained with ethidium bromide.
RNA was checked for integrity in an Agilent Bioanalyzer 2100 with the RNA 6000 Nano Kit (Agilent Technologies, Foster City, CA).
All RNAs were checked for integrity using the 2100 BioAnalyzer (Agilent Technologies) and quantified using a ND-1000 spectrophotometer (NanoDrop Technologies).
The eluate was concentrated by ethanol precipitation, the mRNA resuspended in water, checked for integrity by agarose gel electrophoresis and quantified by spectrophotometry.
Total RNA was subsequently checked for integrity via agarose gel electrophoresis and quantified via UV-VIS spectrometry. 1 µg total RNA was used for cDNA synthesis using the RT2 first strand kit (SA biosciences) according to the manufacturer instructions.
Total RNA was checked for integrity using the RNA LabChips on the Agilent Bioanalyzer 2100 (Agilent Technologies Inc., Palo Alto, CA, USA) and the concentration was determined on a Nanodrop spectrophotometer (Peqlab, Erlangen, Germany).
DNA was checked for integrity by using mitochondrial DNA primers.
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