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To check for expression of APJ in human AVICs, western blotting and immunohistochemical method were performed.
These primers were also used to check for expression of genes involved in ethylene synthesis and signaling in dry wild-type (Col) and fus3 immature seeds.
RNA was extracted from a subset of transformed roots for genes listed in Additional file 2: Table S2 to check for expression of the Arabidopsis gene by PCR.
After reaction, remove 1 μl and add it to 3 μl 2X Laemmli buffer for analysis to check for expression of the protein.
To check for expression of histidine-tagged proteins, 1 ml of cell culture at a cell density of D590 of 1.0 was harvested by centrifugation for 2 min at 17000 g.
We next selected three representatives from the Ranunculales, which is close to the ancient duplication event at the base of the core eudicots, to check for expression in vegetative tissue.
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In addition, we found that after each passage, harvested bacteria must be cloned and checked for expression of the bioengineered gene to counter selection in favor of antigen loss mutants.
Transfected cells were checked for expression of GFP by fluorescence microscopy and harvested using a cell scraper.
Individual clones were checked for expression of the NhttEGFP transgene by microscopy and Western blot analysis following induction with CuSO4.
We also checked for expression of PSD-95 which is a post-synaptic density protein, important for formation of functional synapses.
After retroviral infection and selection with puromycin, the polyclonal populations of knockout cells reconstituted with MEKK1 were checked for expression levels in comparison to the DT40 cell line by western blot (Figure 5A, left panel).
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