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The log files can be examined with the program Tracer [ 50] to check for efficiency of the sampling (ESS), and convergence between independent runs.
Proteins were transferred to a nitrocellulose membrane, which was treated with the MemCode Reversible Protein Stain Kit (Pierce , Thermo Scientific to check for efficiency of protein transfer and for use as a loading control.
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They do not avoid heuristic arguments, and are often checked for efficiency by numerical experiments.
All primer/probe pairs were checked for efficiency of amplification using reverse transcribed rat bone total RNA.
This allowed us to check for the efficiency of Cre recombination by analyzing the expression pattern of β-galactosidase expression.
For stable transfections, 300 000 HeLa cells were seeded in 10 cm dishes and co-transfected using FuGene with 6 µg of the plasmid of interest and 0.5 µg pEGFP-N1 to check for transfection efficiency.
A calibration step of the experiment was used to check for PCR efficiency.
Raw sequence data were first aligned against the Ectocarpus siliculosus rDNA sequences to check for depletion efficiency (rRNA contamination was estimated approximately 0.55% for female and 0.12% for male library) and then to the Ectocarpus genome.
Cells to be imaged were divided into two parts, trypsinized and replated – one set fixed 48 h after siRNA treatment, permeabilized and stained for immunocytochemistry, whereas another set lysed to check for knockdown efficiency biochemically.
Cells to be imaged were divided into two parts, trypsinized and replated after 48 h – one set fixed another 24 h after this, permeabilized and stained for immunocytochemistry, while the second set lysed to check for knockdown efficiency biochemically.
To check for scale efficiencies via the credit volume, we utilize the logarithm of the EAD (EAD(^{mathrm{LN}})) which is EUR 286,000 in our sample.
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