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Usually, 20 isolates are sequenced from each library and the crossover positions are plotted to check for adequate distribution.
Nasal swabs were inoculated on Columbia blood agar plates with 5% sheep blood to check for adequate sampling and subsequently enriched in Mueller-Hinton broth containing 6.5% NaCl.
In addition multiplex I (See Table 1) included a primer/probe set for detection of human β-globulin DNA to check for adequate sample collection and preservation and isolation of DNA of sufficient quality.
The kits include probes for each exon of the gene in question (except BRCA2 exons 5, 6, 23 and 26), probes for a number of control regions across the genome, plus further controls to check for adequate quality of test DNA and efficient ligation.
To check for adequate convergence and confirm a satisfactory effective sample size (ESS > 200), Tracer v. 1.8., LogCombiner v. 1.8, and TreeAnnotator v. 1.8 were used to combine and summarize trees files, obtain a maximum clade credibility consensus tree, and calculate 95%% credibility intervals [ 82].
Pull the radiator cap to check for adequate flow or leaks before it gets too hot.
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Yeast functions best in a slightly acid medium, and the prepared grain mash, fruit juice, molasses, or other mixture must be checked for adequate acidity (pH value).
Preventive strategies of DC dysfunction include ultrasound guidance for a higher chance of DC placement [5], checking for adequate flow immediately after placement, flushing each DC lumen with saline at the beginning and end of each RRT session, anticoagulant locking followed by careful clamp closing, and checking the correct position with x-rays.
The clean up workers need respirators with vapor cartridges (and need to be checked for adequate fit).
Each sample and hybridization underwent a quality control evaluation checking for adequate scaling factors (1 3 for all samples), percentage of probe sets reliably detected (between 40 60% present call), and optimal 3'/5' hybridization ratios for the housekeeping genes (e.g., GAPDH), poly(A) spike-in controls, and the prokaryotic controls (bioB, bioC, bioD and cre).
The generation vs. log probability plots were checked for adequate sampling and potential scale reduction factors for convergence.
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