Sentence examples for check digestion from inspiring English sources

Nonselective PCR was performed to check digestion and ligation reactions.

Check whether digestion is complete on agarose gel.

Digest 500 μg of L8G515S plasmid with 100 units of BamH1 (New England Biolabs) for 2 hours at 37°C in 500 μl in 1 X New England Biolabs (NEB) buffer 2. Check whether digestion is complete by loading a small aliquot on agarose gel Add: 75 μl of 0.4 mM biotinylated dATP, 75 μl of 1mM dCTP, dTTP and dGTP, 25μl NEB buffer 2, 100 units Klenow polymerase (Invitrogen) and H2O to a final volume of 750 μl.

The orientation of the subcloned fragment was checked by digestion with two restriction enzymes, Sal I and Xho I, sites that are present in the ORF of OsIDS1.

Each insertion was checked by digestion with appropriate restriction enzymes, PCR, and sequencing.

The dilutions were loaded on to a Prionics®-Check WESTERN™ digestion plate, and the test was conducted as per the manufacturer's instructions.

The integrity of the recombinant BACs were checked by digestion with Hind III (data not shown) and the recombination was confirmed by sequencing of the PCR product (data not shown).

The integrity of the mutant BACs were checked by digestion with Hind III (data not shown) and the correct recombination was confirmed by sequencing of the PCR product (data not shown).

The construct was checked by digestion and sequencing.

We also checked the digestion efficiency by incubating the NNKOAc-treated CT-DNA with the enzymes for ≤48 h.

The insert sizes were checked by digestion with EcoRI, sequenced by Sanger sequencing, and assembled using Sequencher 4.10.1 using non-repetitive flanking sequences to guide alignment.