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The reaction was terminated after a final extension at 68°C for 5 min. Control reactions were done using the same procedures, but without DNA added to check contamination of the PCR reaction.
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Scientists have developed a new test to check for contamination.
The city said it was shutting its water system for four days to check for contamination from an explosion at a chemical plant in nearby Jilin.
Quality control measures were conveyed to check the contamination and the reliability of the results obtained.
PCR analysis was also carried using VirD gene-specific primers to check the contamination of hairy roots with residual Agrobacterium.
To check the contamination of genomic DNA, reverse transcription was omitted in the negative control.
To check for contamination of DNA, negative controls were included by omission of the reverse transcriptase.
The blank sections were tested by S14 PCR to check for contamination during sectioning.
No-template-controls were used for each PCR set to check for contamination.
We ran negative controls that contained no DNA template with each reaction to check for contamination.
Positive and negative controls were used to check for contamination and unspecific amplifications.
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