Before conducting further analysis, we checked assembly data of BAC contig, and found no apparent errors (data not shown).
However it is important to note, that despite these careful quality checks, assembly of some regions that have a particularly high frequency of repeats should remain provisional.
Eventually the unique structure was formed with the monomer cone valve integrally embedded into the check valve assembly, as shown in Fig. 3.
The field hand-over test of equipment is required to check the assembly quality of equipment following field installation and before being put into operation to evaluate the equipment performance.
SL, MS and MPK contributed mapping information and examined linkage data for all autosomes to quality check the assembly.
To check the assembly result, nine partially overlapped fragments covering the entire C. oliveri cp genome were amplified and sequenced.
The PCR to check the assembly was conducted using the TaKaRa Ex Taq system and the primer pairs used are listed in Table 1.
ZW carried out most of the experiments (including PCR, sequence check and assembly) and initial annotation; XK initiated, led the research, and supervised all laboratory work; WS participated in data analyses and made all figures.
Check the assembly at this point to make sure it is square.
Screw the check valve assembly from the kit onto the pump.
