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The same calculation was then applied to the subsequent 21 controls and 24 AD patients to check assay performance in an independent patient group.
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Proteolytic activity in the culture medium was assayed using the P-check assay.
Protease activities of culture medium samples were measured using the P-check assay at pH 2.7 according to the supplier's instructions (Jena BioScience).
Since no gold standard exists for this bioassay, participation in specifically designed proficiency panels is a prerequisite for checking assay performance and provides a point of comparison to other laboratories testing the same samples for the same reactivity, following their lab-own protocol [ 8, 9].
RNA integrity was checked using Nano Chip Assay by Bioanalyzer 2100 (Agilent technologies, Böblingen, Germany).
Following the synthesis, the purification and the structural checking, antibacterial assays revealed a strong activity against gram-positive and gram-negative bacteria including human pathogenic bacteria such as Staphylococcus aureus and some marine bacteria of the genus Vibrio.
Similarly, Gopinath et al. (2014) constructed five different microbial consortia and the compatibility of the consortia to degrade organic waste was checked by enzyme assays of four substrates (starch, cellulose, casein and tributyrin).
The specificity of the used antibodies has been checked using competition assays (Figure 3).
However, fully functional IL-8 (as checked in migration assays) was completely incapable of decreasing T-cell allostimulation as mediated by DC.
We carefully evaluated these assays, checked the concordance of duplicate samples, and tested for deviations from Hardy-Weinberg equilibrium.
Cell infection was checked by indirect immunofluorescence assays (IFA) using mouse ascitic fluid directed against CHIKV.
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