Hyperglycemia was confirmed 48 hours (h) later using Accu-Check Advantage II blood glucose test strips (Roche, Mannheim, Germany).
Blood glucose levels greater than 250 mg/dL, measured by an Accu-Check Advantage glucometer (Boehringer Mannheim Corporation, Indianapolis, IN), indicated the development of diabetes.
Blood was collected by tail nick at 0, 15, 30, 60, and 90 min postinjection, and blood glucose was measured immediately using an Accu-Check Advantage meter (Roche Diagnostics, Burgess Hill, West Sussex, UK).
Seventy-two (72) hours after, their blood glucose levels were measured using the Accu-check Advantage II Glucose meter (Roche Diagnostics Co., USA) and rats with fasting blood glucose ≥ 15 mmol/L were considered diabetic and included in the study.
This test comprises 6 blood glucose measures using test strips (Accu-check Advantage, Roche, Mannheim, Germany) at baseline (before insulin administration), 4, 8, 12, 16, and 20 minutes post-insulin application.
After 72 h of STZ injection, fasting blood glucose (FBG) was measured using the Accu-check Advantage II Glucose meter (Roche Diagnostics Co., USA) and rats with FBG (mmol/L) ≥15 ≤ 25 were considered diabetic and used in the experiment.
Briefly, mice were fasted for 5 h, and baseline blood glucose levels were measured with an Accu-Check Advantage blood glucose meter (Roche, New Jersey, USA) using blood collected from the tail vein.
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The agreement was intended to check Microsoft's advantage of bundling its browser into Windows, a practice that a Norwegian browser maker, Opera, challenged in a complaint to the European Commission in December 2007.
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