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In particular, the rational design of an MCF involves: (i) product selection, (ii) de novo biosynthetic pathway identification (i.e., rational, heterologous, or artificial), (iii) MCF chassis selection, (iv) enzyme engineering of promiscuity to enable the formation of new products, and (v) metabolic engineering to ensure optimal use of the pathway by the MCF host.
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Use of the Cre-loxP recombination system, which allows repeated use of a selection marker (Güldener et al. 1996), facilitated construction of the wild-type chassis cell.
Build car chassis.
scrap of chassis.
Build a chassis.
Engine man, chassis man, driver".
The chassis alone cost $50,000.
The Mustang has an all-new chassis.
Fiat is making chassis' for Kaiser.
(2 Special rule applicable to chassis and bodies.
§ 1066.220 Linearity verification for chassis dynamometer systems.
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