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Two of the most intense precursor ions that were doubly or triply charged were selected from m/z 350 to 1990.
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The values of magnetization vector and surface charge were selected as follows: M=570 kA/m; σ=2.5×10−5 C/m2.
Values for the magnetization vector and surface charge were selected in the following way: M=570 kA/m; σ=2.5×10−5 C/m2.
These charges were selected, because the B3LYP theory level, 6-311G baset set and NPA proved to be very suitable for EEM parameterization [37, 38, 40].
Albumin was fragmented using cyanogen bromide and six albumin-derived peptides with different numbers of electric charges were selected and synthesised.
Target ions with +2 to +5 charge were selected for sequencing once they reached a threshold of 100 counts per second.
Ions >+1 charge were selected for fragmentation by collision-induced dissociation, with up to 20 fragmentation spectra (MS2) collected per MS1.
Five peptides containing different combinations of positive and negative charges were selected and synthesised, each composed of 16 amino acid residues.
The 12 most intense parent ions excluding singly charged ions and ions with unassigned charges were selected for higer-energy collisional dissociation (HCD) fragmentation with a normalized collision energy (NCE) set to 28%.
At low QD potentials, particles with a large number of charges are selected, with the number of charges limited by the Rayleigh limit.
Dynamic background subtraction was used prior to ion selection; the four most abundant doubly or triply charged ions were selected for the product ion scans.
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