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One of the reasons for this linker length dependence could be the FLAG tag sequence (DYKDDDDK), which possesses a negatively charged sequence.
Likewise, the representatives used in this study (aSyn, Aβ, htt, and PrP) do not share any charged sequence similarity.
The positively charged sequence of the JM region suggests that electrostatic interactions are essential for both membrane binding and release.
Among them, only GDHs from subsets Ref, G, I2 and I3 contain a Ser residue equivalent to Ser205 (followed in addition by the same positively charged sequence DFD).
Did this negatively charged sequence act as a barrier to receptor activation which could be overcome by binding of the bivalent cytokine ligand?
However, we demonstrated that ECD of the triply charged nitrated peptides resulted in some singly charged sequence fragments, which may be the products of secondary electron capture.
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The first time they do this for an audience, she's hidden behind a sheet, and it's one of the most sexually charged sequences ever filmed, full of danger and audacity.
The occurrence of charged sequences along its structure provides an indication that such coatings may be formed through electrostatic interactions.
Thus, the positively charged C-terminus region has unique properties compared to the negatively charged sequences.
McLaughlin and others have shown that such highly positively charged sequences can bind to negatively charged membrane surfaces and serve to sequester the highly negatively charged phosphoinositide, PIP2.
Only in case of very long stretches (5 residues or more) we have considered equally charged sequences as CCXCC or CXCCCC where C means Charged and X is any not charged amino acid.
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