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It is suggested that electrostatic attraction of negatively charged bacteria to the positively biased photoanodes leads to the enhancement observed.
Thus, in qualitative terms, less charged bacteria (i.e., E. coli) will associate more easily with the α-HL nanopore.
Particularly, crystalline TiO2, which presented a predominant acidic nature, was more attractive for the adhesion of the negatively charged bacteria.
The negative electric potential on the trans side of a lipid membrane containing a single α-HL nanopore drives negatively charged bacteria into the nanopore.
Thus, a negative charge distribution on this region reflects as an electrostatic barrier for the electrophoretically driven, negatively charged bacteria towards the α-HL (Fig. 6).
If negatively charged bacteria (Hubbuch et al. 2006) are also seen as particles their charge could be reversed by Mg2+ ions present in the cultivation medium and they could be flotated by negatively charged produced rhamnolipids.
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It has been suggested that this feature ensures that the antimicrobial peptides will only be "activated" into the cytotoxic form in the presence of the target cell membrane, in this case a negatively charged bacterium, and will not indiscriminately damage nontarget host cells [ 17].
In contrast to LL-37, however, no marked helix induction was detected for the PrP-derived peptides in presence of negatively charged (bacteria-mimicking) liposomes.
This effect appears to be profound for weakly and strongly charged hydrophilic bacteria and may contribute to differences in observations between laboratory experiments versus field-scale investigations particularly if the groundwater pH remains subneutral and Fe oxyhydroxide phases exist.
Although found to have slightly more charged proteomes, bacteria seem to have a decreased content of negative charges.
If highly charged airborne bacteria pass through an electric field generated by a plastic object, then they are likely to move either towards, or away, from the surface depending on the polarity of the charges involved.
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