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However, control over its charge state lags far behind.
The first section selects the most abundant charge state.
Fig. 4 Charge state of different amino acids.
Positive values indicate charge state while negative indicate discharge.
QDs captured a photon change their charge state.
For partial tryptic, Xcorr ≥4.3 for charge state +2 and ≥4.7 for charge state +3.
Specific filter criteria for this study to achieve this level of confidence include DelCN ≥0.1 coupled with Xcorr of ≥1.6 for full tryptic charge state +1, ≥2.4 for charge state +2, and ≥3.2 for charge state +3.
The peptide identifications obtained were filtered in Bioworks with four filter criteria: ΔCn >0.08, Xcorr >1.5 for charge state 2+, Xcorr >3.3 for charge state 3+, and Xcorr >3.5 for charge state 4+ [4+].
Criteria for filtering SEQUEST search results, were retention of: 1) fully tryptic peptide identifications provided ΔCn >0.1 and XCorr >1.5 for peptides assigned 1+ charge state, >1.9 for 2+ charge state, and >2.9 for 3+ charge state; and 2) partially tryptic peptides provided ΔCn >0.1 and XCorr >3.1 for peptides assigned 1+ charge state, >3.8 for 2+ charge state, and >4.7 for 3+ charge state.
PSMs were segregated by charge state.
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Charge state screening was used to select ions with at least two charges and to reject ions with unassigned charge state.
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