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Change in pH facilitates changes in the net charge of protein and also affects the partitioning behaviour of the protein.
When pH is lower than the isoelectric point of proteins, the net charge of protein is positive, while the reverse case is negatively charged [8, 9].
They have revealed that the composition of hard protein corona was changed noticeably by applying both irradiation and thermal heating while there was no significant effect on the surface charge of protein corona.
In consistent with lower transcriptional level, proteasome, the complex in charge of protein degradation, is negatively regulated in the long-lived mutants.
In brief, the method uses hydrophobicity and net charge of protein sequence segments in order to distinguish disordered from ordered regions.
For example, the net charge of protein is shown to be the only factor that correlates with the reduction potentials of different HiPIPs.
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In turn, protein concentration can be altered by changing the charge of the proteins: higher electrostatic charge of proteins causes them to repel each other more, resulting in a less densely packed group of proteins and lower refractive index.
Some proteins with horizontal patterns could for instance, indicate phosphorylations, changing the charge of proteins as observed in the train of protein spots assigned to selenium binding protein.
The fluorescent dyes have been designed so that they do not alter the charge of proteins or impart significant electrophoretic mobility differences to identical proteins.
In the narrow selectivity filter of each pore, Cl− are bound to several sites through electrostatic interactions with helix dipoles and the partial charges of protein residues.
The charges of protein atoms were originated from AMBER parm99 (Wang et al 2000; Case et al 2004).
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