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ERα status was determined at the protein level by using biochemical methods (Dextran-coated charcoal method until 1988 and enzyme immunoassay thereafter) and was confirmed at mRNA level by real-time RT-PCR.
ER levels were measured by the dextran-coated charcoal method, as previously described [ 14].
Estrogen receptor (ER) levels were measured using the dextran-coated charcoal method as previously described [ 21].
Oestrogen receptor (ER) content were determined biochemically using the dextran-coated charcoal method in Tokyo and Oxford.
Oestrogen receptor binding was determined by a dextran coated charcoal method and median follow up was 11 years.
Assays for EGFr (two point radioreceptor assay) and oestrogen receptors (ER) (dextran coated charcoal method and an immunohistochemical method) were performed on 20/22 patients.
ER status was measured by the dextran-coated charcoal method and tumours with ER values >10 fmol/mg protein were considered ERP.
The DNA bank was created using frozen, histologically proven invasive breast cancer specimens that were primarily handled for ER testing by the dextran charcoal method.
Estrogen receptor (ER) and progesterone receptor (PR) status was determined at the protein level by using biochemical methods (dextran-coated charcoal method or enzyme immunoassay) until 1999 and then by immunohistochemistry.
ER status was determined at the protein level by using biochemical methods (Dextran-coated charcoal method until 1988 and enzyme immunoassay thereafter) and was confirmed at mRNA level by RT-PCR.
The stored DNA was isolated (see below) from frozen, histologically proven and macrodissected invasive breast cancer specimens that were handled primarily for ER and PR testing by using the dextran charcoal method as previously described [ 22, 23].
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