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The thickness is based on the mucus containing beads, and as the mucus in IL-10−/− mouse colon is very penetrable, this results in a smaller value than in figure 3B where the mucus thickness is based on charcoal binding to the mucus surface.
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From 1,211 breast cancers, 15 oestrogen receptor (ER) negative-progesterone receptor (PgR) positive breast cancers by conventional dextran coated charcoal steroid binding assays in cytosol were reassessed using Elisa techniques with monoclonal antireceptors antibodies in the cytosolic and nuclear fractions, and immunocytochemistry on cryostat sections.
ER positivity was defined as a dextran-coated charcoal ligand-binding assay (LBA) result of more than 10 fmol/mg protein [ 19].
A larger value of about 0.02 has been reported by several other research groups: Using equilibrium dialysis, Altmayer et. al [ 17] reported values ranging from 0.014 to 0.026 and Servin et. al. [ 18] reported an average value of 0.022; while Mazoit and Samii [ 19] report an average value of about 0.02 using a charcoal co-binding technique.
To address binding to charcoal at later time points, microplates containing compounds in PBS containing or not containing 0.4% activated charcoal (wt/vol) were incubated 18 to 24 h at room temperature.
A multi-method approach to the research of pigments and binding media, charcoal, and cave sediments was used to elucidate the technologies, chronologies and processes of indigenous art and artists.
About 15%% (1.5 g) of 10 g oligosaccharides in the hydrolysate were lost due to co-elution with monomeric sugars and irreversible binding to the charcoal matrix.
Tumors were characterized for ER and PR status by immunohistochemistry (IHC), ligand-binding dextran-coated charcoal assay or reverse phase protein lysate array (RPPA).
Breast cancer cytosols classified as ER-positive according to [H]oestradiol-binding assay (dextran-coated charcoal [DCC]) were subjected to hydroxylapatite adsorption.
To determine whether serum proteins affected the bioavailability of natural estrogens compared to xenoestrogens, albumin, sex hormone binding globulin (SHBG), or charcoal-stripped serum were added to the YES system and beta-galactosidase activity assayed.
Our present study was more sensitive in terms of binding affinity and measurements with charcoal compared to 99mTc-labeled resin method.
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