This chapter describes methods for growing, purifying, counting, and characterizing viruses.
We envision mesofluidic nanoparticle manufacturing devices, based on the initial design here, capable of self-assembling, purifying, concentrating, and characterizing virus-based nanoparticle formulations with a single injection of precursors.
In the past, investigations of shipboard outbreaks of viral gastroenteritis were limited by the lack of adequate molecular methods for detecting and characterizing viruses (7 ).
Next-generation sequencing (NGS) was used to characterize virus strains molecularly.
We designed primers that would: (1) amplify regions <150 bp due to the likelihood that the CAS samples were degraded; and (2) discriminate between previously <span class="lh lhl">characterized virus sequences.
This method was applied to 17 previously characterized virus isolates [ 11] and 2 newly identified ones.
Different molecular techniques were used by participating institutes to characterize virus from outbreaks in 2000.
These programs rely on serological assays to characterize virus subtypes, establishing seroprevalence and evaluating vaccine efficacy [ 8, 11, 17].
In its sensitive detection mode (see the user's manual at [ 30]), VirusFinder runs VERSE to characterize virus integration loci.
To investigate arenavirus in Zambia, we characterized virus from the kidneys of 5 arenavirus RNA positive rodents (Mastomys natalensis) among 263 captured.
Moreover, from our previous experience with sampling groundwater, we concluded that multiple water samples over time would be necessary to accurately characterize virus exposure.
