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After characterizing the functionality of the individual SIM components in TX-TL, we transfer the final RNA-SIM circuit to E. coli, and show that this network dynamically stages the expression of two fluorescent reporter proteins in vivo, solidifying the use of TX-TL for engineering RNA genetic circuits.
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Moreover, previous studies have generally taken a "generic" or theoretical approach to characterizing the functionalities of EMR systems, and have focused on specific features, such as electronic communication, or on individual functionalities, such as the monitoring of chronically-ill patients.
Other technologies like cell-based assays, biacore, and homogeneous mobility shift assays can characterize the functionality of anti-drug antibodies; however, the question arises whether characterization of the antibody binding activity is required, when this can be easily answered with a simple ELISA test due to the fact that the immune response detected by ELISA is neutralizing.
The objective of RBS is to characterize the functionality of each robot for realizing an specific task.
Interfacial mechanisms associated with TLM elements helped to quantitatively characterize the functionality of the different LiCoO2/AC ratios present in the electrode compositions.
The results of a number of simulation experiments with simple parts are reported, both to characterize the functionality of the scheduler and to illustrate the operation of the entire software system for automatic generation of robot programs for painting.
Several operating variables were selected in order to characterize the functionality of this coagulant: the coagulant dosage, coagulating temperature, pH level and the initial pollutant concentration (so-called charge).
To characterize the functionality of the HA spike on mammalian VLPs, hemagglutination assays were performed (Fig. 4E).
Here, we characterized the functionality of a highly abundant type of miR-101 variant that differs in the seed region.
Ultimately, the CRISPR-Display system enables precision ectopic targeting of RNA and ribonucleoprotein to loci of interest in order to fully characterize the functionality of the RNA.
To characterize the functionality of those addictive drugs' targets, we performed an enrichment analysis of KEGG canonical pathways using the online tool Web-Based Gene Set Analysis Toolkit (WebGestalt) [ 16].
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