The CBB R350-stained 2-DE gels were imaged with a Versdoc 4000 scanner (Bio-Rad), and the spot patterns were characterized using the PDQuest software (ver. 8.0.1, Bio-Rad).
The GC-MS peaks were identified by comparison with data found in the literature and characterized using the library search software of Shimadzu Class-Vp (Wiley 138 and Nist 98 databases).
Candidate TFs responsible for differential expression profiles of the immediate early genes were characterized using the Promoter Analysis and Interaction Network Toolset (PAINT) software [ 21, 22].
After incubation with Hoechst 33342, approximately 60% of the cells were in G2 phase, as characterized using ModFit software to analyze the flow cytometry data.
With the help of the LMW-GS genes available from the Aroona NILs and some other wheat varieties [ 10, 13, 14, 17, 18], LMW-GS genes were characterized using Lasergene software (DNAStar; http://www.dnastar.com/), ClustalW2 (http://www.ebi.ac.uk/Tools/msa/clustalw2/), and MEGA 5 software [ 52].
All selected sensors were first individually calibrated and characterized using commercially-available software and measurement tools, they are then collectively interfaced to a custom-designed flexible radio-frequency transmitter device for real-time performance measurement within a clinical-grade wound bandage.
To analyze the distribution of the remaining fragments, three images of a 0.1 mL sample of the fragments were imaged using a LEICA DM light microscope (Leica Microsystems Ltd ,Heerbrugg, Switzerland) at a magnification of 2.5 times, and root debris lengths characterized using Image J software (Rasband and Bright, 1995).
The 10 SL sites were characterized using an open-source software multiscale modeling of macromolecular systems (MMM, (Polyhach et al., 2011 ) based on the crystal structure data of α-actinin-2.
The groups were characterized using means ± SD and compared by Student's t test for continuous variables and χ test for discrete variables using SAS 9.1.3 software.
