Exact(1)
SCCmec type III variants were characterized using a multiplex PCR method that has been validated for detecting structural variations of type IIIA, which has lost the pT181 integrated plasmid, and type IIIB, which has lost pT181 and SCCmercury [45].
Similar(59)
β-lactamase producing plasmids were characterized using multiplex PCR (employing specific forward and reverse primers [ 43]) followed by elecrophoresis to identify specifically sized PCR products, thereby differentiating three common plasmids: Asian (958 bp), African (1191 bp) and Toronto (650 bp).
The T. cruzi isolated obtained was characterized using mini-exon multiplex PCR, as described by Fernandes et al. [ 30].
Multiplex OLA sensitivity was characterized using template DNA sequences consisting of an abridged subtype B HIV-1 Pol gene consensus and six different permutations of drug resistance mutations.
Therefore, the aim of our study was to better characterize the profiles of cytokines and other inflammatory mediators in the serum and cerebrospinal fluid (CSF) during IMD using a multiplex assay.
Using a multiplex PCR assay, X. oryzae pv.
Cytokines were measured using a multiplex sandwich-ELISA assay (Aushon Biosystem, Billerica, MA).
SCCmec type assignment was performed using a multiplex PCR method, as described previously [22].
cDNA was synthesized from total RNA using a Multiplex RT for Taqman array kit (Applied Biosystems, Foster City, CA).
Genotyping was performed using a multiplex protocol (see Supporting Information).
Molecular serotyping was completed using a multiplex PCR scheme [ 61].
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