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To further delineate the impact of 11 selected candidates on normal hematopoiesis, we characterized their effects in gain- and loss-of-function studies using CD34+ hematopoietic stem and progenitor cells (HSPCs).
To test these substitutions' functional importance, we introduced the derived states individually into Anc-gkdup and characterized their effects on guanylate kinase activity, Pins binding, and spindle orientation.
We will be able to distinguish among them in individual cases only when we have identified the causative alleles and characterized their effects.
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To circumvent this problem, we used lower concentrations of his10GFP-Lpd (850 1250aa) oligomers purified by size exclusion chromatography and characterized their effect on VASP barbed end processivity in the single actin filament TIRF assay.
Several measurement techniques were already used to characterize their effects and the degradations are mainly attributed to carbon oxidation: a decrease of the cathode active area is commonly observed after start-up/shut-down cycles.
Finally, sound pressure level (SPL), subjective loudness and pain intensity were measured to further characterize their effects on the subjects.
Here we study miRNAs with conserved binding sites in the Mitf 3'UTR sequence and characterize their effects on Mitf using a reporter construct.
Although it is impossible to control for all pre-analytic variables, efforts to characterize their effects are under way.
Our aim is to identify the genetic loci (QTL) that influence the function-valued phenotype and to characterize their effects over time.
Here two HDACi included in clinical trials have been tested, suberanilohydroxamic acid (SAHA) and valproic acid (VPA), to characterize their effects on glioma cell growth in vitro and to determine the molecular changes that promote cancer cell death.
To conduct such experiments, it would be necessary to purify the mutant H NS proteins and characterize their effects on bridging using AFM before using them to test hypotheses about the EMSA assays.
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