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The Bloomberg officials had characterized the requirement as an important safety precaution and cited it as the rationale for reassigning a local engine company's commander whose firefighters had not inspected the bank building in the months preceding the fire, in which two firefighters died.
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He characterized the requirements as somewhat unrealistic but said the process was established under a 2007 national law.
The first evidence that T cells can recognize cancer was based on the description by Wolfel et al. [ 1] of tumor infiltrating lymphocytes (TILs) that could kill autologous tumor cells following a human leukocyte antigen (HLA -restricted pattern and by Kawakami et al. [ 2] wHLA -restrictedd the requirements for HLA restriction.
Here we characterize the requirement of Cul-5, a key component of a subgroup of Cullin-RING-type ubiquitin E3 ligases, in Drosophila oogenesis.
To further characterize the requirement for TCR signaling, we infected activated mouse T cells and re-plated them without anti-CD3 stimulation.
We also used 3 independent in vivo models to precisely characterize the requirement for WASp in the central versus peripheral B-cell compartments.
To further characterize the requirement for Stat3 in MCF10A-Ras cells, we utilized a Matrigel assay to examine growth in three-dimensions (3-D).
To further characterize the requirement of fss/tbx6 in the formation of central dermomyotome cells and segment boundaries, we induced a pulse of Tbx6 expression at varying times during the segmentation period, and monitored segmentation and the development of the dermomyotome.
To more carefully characterize the requirement for RIPK3, we next used genetic approaches to reconstitute RIPK3 expression in the RIPK-deficient, death-resistant HEY cells and to suppress RIPK3 expression in the death-inducible OVCAR3 cells.
In order to characterize the requirement for these six lysines in SopB ubiquitination, site-directed mutagenesis was used to create lysine-to-arginine substitutions in the pCMV-Myc-SopB plasmid.
Based on the results of field comfort surveys an adaptive comfort model for children and teenagers was developed and compared to ASHRAE's model for characterizing the requirements of thermal comfort among the students using naturally ventilated classrooms.
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