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As the majority of these ORFs have no homologs with characterized sequence or protein structure, traditional sequence-based approaches cannot be applied to deduce their biological function.
We also found intraspecies polymorphism within P. cynocephalus anubis TRIM5α, as there were one synonymous and three nonsynonymous SNPs between the newly characterized sequence and the published sequence [26].
Most characterized sequence superfamilies within this assemblage appear to bind small molecules or soluble ligands.
Identified RAYTs were checked to contain previously characterized sequence motifs peculiar to RAYTs [ 14].
DNA methylases have well characterized sequence signatures not present in these proteins.
To further characterized sequence annotation, all unigenes were searched against COG database to analyze phylogenetically widespread domain families.
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The pattern of heteroduplexes from each reaction were then compared to the mobilities of previously characterized, sequence-verified Mane MHC class I clones using DAx data acquisition and analysis software (Van Mierlo Software, Eindhoven, the Netherlands) [9], [19].
Similarly to clade I, the biochemically characterized sequences of clade II belong to a single FAE type.
In addition, the number of incompletely characterized sequences in the public databases has been growing at a rapid rate, especially in GenBank.
Five TG2 peptide substrates that were predicted to bind to HLA-DQ2.5 did not contain previously characterized sequences of T-cell epitopes.
Characterized sequences were derived from UK isolates of S. nodorum [ 35].
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