We specifically selected this region to have a well characterized reference sequence to compare our experimental results to.
In order to further classify the 42 genotype 4 HEV strains, phylogenetic analysis was performed including 16 well characterized reference sequences [16].
Specificities were verified using previously characterized reference sera.
CT and DSC have the advantages of practicality, speed and reproducibility and do not require a previously characterized reference standard.
For organisms with well characterised reference genomes, such as Arabidopsis, this requirement is easily met, while organisms lacking a well characterized reference sequence present numerous challenges.
Three well characterized reference viruses were used, including two classified as genotype 3: Nakayama and SA14-14-2, and one from genotype 1: TVP-8236.
In this study, PCR was evaluated for its efficacy in the detection of fungal pathogens in corneal scrapings using characterized reference strains.
Besides instrumentation, the absolute quantification of nanoparticles, the validation of the analytical method of choice and the calibration of the analytical equipment require the availability of appropriately characterized reference materials.
Improving the conclusiveness of patient-specific iPSC-CM models with approaches to address line-to-line variability and genetic background variations via CRISPR-generated isogenic controls, or fully sequenced and characterized reference stem cell lines (either iPSCs or ESCs).
In this study, the 454 GS-FLX platform was utilized due to its longer read length, which enables high-quality de novo assembly of the transcriptome without a characterized reference genome [ 39].
In order to assess the performance of the different de novo TAs as scaffolds for RNA-Seq expression analysis, we compared the use of a TA as a mapping scaffold to that of a predicted gene set from the well characterized reference genome of H. sapiens.
