Besides instrumentation, the absolute quantification of nanoparticles, the validation of the analytical method of choice and the calibration of the analytical equipment require the availability of appropriately characterized reference materials.
These findings demonstrate the advantages of using the dPCR method to characterize reference materials.
A well-characterized reference material for CD4+ cell counting could highlight issues with different approaches and instruments combinations potentially under or overestimating CD4+ cell counts.
Specificities were verified using previously characterized reference sera.
Recently, the National Institute of Standard and Technology (NIST) has – together with other institutions – prepared and characterized standard reference material (SRM) 3276 delivering carrot extract in oil that provides, inter alia, certified values for trans-α-, trans-β-, and total β-carotene [ 84], including expanded uncertainties.
According to the ISO Guide 34 (http://www.iso.org/iso/home.html), validated analytical methods should be applied to characterize the reference material, during its development and production.
In addition, if each reference is carefully weighed with a microbalance, the latent heat effects of these melts can be integrated to provide calibration data for the calorimeter equation assuming the heat of fusion is well characterized for each reference material used to compute the temperature correction.
Development of characterized genomic DNA reference material panels for clinical chromosomal microarrays.
LC IDMS, an independent DNA concentration measurement approach developed in our earlier study, was used to co-characterize the plasmid reference material with ddPCR.
The NCEH used its Roche E-170 procedure to characterize 3 levels of National Institute of Standards and Technology NISTT) standard reference material (SRM) 3951 (30), which is under development.
A reference material should be well characterized and include an estimate of uncertainty; that is, the range of values within which the true value is asserted to lie.
