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Cryopreservation could provide us with a welcome alternative to safely store genetically well characterized lines.
We further characterized lines we intended to use in behavioral experiments.
Among the 25 molecularly characterized lines, four are integration into exons and therefore null alleles.
The 2 most extensively characterized lines, NC-m4 and NC-m6, have been grown up to 31 and 30 passages, respectively (≥90 population doublings).
Our aim was to develop genetically characterized lines of the salt-tolerant Salicornia (marsh samphire) and Sarcocornia (shrubby Swampfire) as new crop plants.
Our aim was to develop genetically characterized lines of Salicornia and Sarcocornia for further breeding and to determine optimal cultivation conditions.
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Therefore, a previously characterized line of mouse mTS cells [4] was taken as positive control.
The authors used a well characterized line of Arabidopsis (CL37) previously engineered to express the castor hydroxylase.
The detection of carmine was also supported by the SERS spectrum, in which the characterizing lines of carminic acid arose following HF hydrolysis (Figure 2).
Conversely, of 69 characterized control lines, only 10% (seven lines) showed a neural-restricted pattern.
(A ) Schematic representation of the feedback inhibition systems required for sterol homeostasis in yeast (left) and mammals (right) previously characterized (dotted lines) and described here (solid lines).
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